Granule cell precursor proliferation was decreased in the cerebellums of <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5. Bum Jun Kim Daryl A. Scott 10.1371/journal.pone.0087518.g004 https://plos.figshare.com/articles/figure/_Granule_cell_precursor_proliferation_was_decreased_in_the_cerebellums_of_Rere_om_eyes3_embryos_at_E18_5_/911297 <p>A-F. Proliferation assays were performed on cerebellar vermis sections prepared from the cerebellums of wild-type and <i>Rere</i><sup>om/eyes3</sup> embryos and mice at E18.5 (A–B), P0 (C–D) and P3 (E–F) using an anti-Phospho Histone H3 antibody. A-B. The number of mitotic granule cell precursors in <i>Rere</i><sup>om/eyes3</sup> embryos appeared to be lower than that of their wild-type littermates at E18.5. C-F. Mitotic activity of GCPs was comparable between <i>Rere</i><sup>om/eyes3</sup> embryo and wild-type littermate at P0 and P3. G. Phospho-Histone H3-positive cells in the entire external granule cell layer (EGL) were counted and normalized by the area of the EGL (n≥3 with twenty slides containing three sections for each genotype). The number of Phospho-Histone H3-positive GCPs per µm<sup>2</sup> was reduced in <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5 when compared to wild-type littermates (* = <i>p</i><0.03) but was indistinguishable between <i>Rere</i><sup>om/eyes3</sup> embryos and wild-type littermates at P0 and P3. ABL, anterobasal lobe; ADL, anterodorsal lobe. Scale bar  = 200 µm.</p> 2014-01-23 04:44:19 Anatomy and physiology Neurological system Central nervous system neuroanatomy developmental biology Molecular development signaling morphogenesis cell migration Organism development organogenesis embryology Pattern formation genetics Animal genetics gene expression Gene function Model organisms Animal models mouse Molecular cell biology Cell growth precursor proliferation cerebellums embryos