10.1371/journal.pone.0087518.g004
Bum Jun Kim
Bum
Jun Kim
Daryl A. Scott
Daryl
A. Scott
Granule cell precursor proliferation was decreased in the cerebellums of <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5.
Public Library of Science
2014
Anatomy and physiology
Neurological system
Central nervous system
neuroanatomy
developmental biology
Molecular development
signaling
morphogenesis
cell migration
Organism development
organogenesis
embryology
Pattern formation
genetics
Animal genetics
gene expression
Gene function
Model organisms
Animal models
mouse
Molecular cell biology
Cell growth
precursor
proliferation
cerebellums
embryos
2014-01-23 04:44:19
Figure
https://plos.figshare.com/articles/figure/_Granule_cell_precursor_proliferation_was_decreased_in_the_cerebellums_of_Rere_om_eyes3_embryos_at_E18_5_/911297
<p>A-F. Proliferation assays were performed on cerebellar vermis sections prepared from the cerebellums of wild-type and <i>Rere</i><sup>om/eyes3</sup> embryos and mice at E18.5 (A–B), P0 (C–D) and P3 (E–F) using an anti-Phospho Histone H3 antibody. A-B. The number of mitotic granule cell precursors in <i>Rere</i><sup>om/eyes3</sup> embryos appeared to be lower than that of their wild-type littermates at E18.5. C-F. Mitotic activity of GCPs was comparable between <i>Rere</i><sup>om/eyes3</sup> embryo and wild-type littermate at P0 and P3. G. Phospho-Histone H3-positive cells in the entire external granule cell layer (EGL) were counted and normalized by the area of the EGL (n≥3 with twenty slides containing three sections for each genotype). The number of Phospho-Histone H3-positive GCPs per µm<sup>2</sup> was reduced in <i>Rere</i><sup>om/eyes3</sup> embryos at E18.5 when compared to wild-type littermates (* = <i>p</i><0.03) but was indistinguishable between <i>Rere</i><sup>om/eyes3</sup> embryos and wild-type littermates at P0 and P3. ABL, anterobasal lobe; ADL, anterodorsal lobe. Scale bar = 200 µm.</p>