%0 Figure %A L. Uhernik, Amy %A Li, Lun %A LaVoy, Nathan %A J. Velasquez, Micah %A P. Smith, Jeffrey %D 2014 %T Characterization of Mct1 expression patterns in cells transfected with full length and deletion mCherry-Mct1 expression constructs. %U https://plos.figshare.com/articles/figure/_Characterization_of_Mct1_expression_patterns_in_cells_transfected_with_full_length_and_deletion_mCherry_Mct1_expression_constructs_/902295 %R 10.1371/journal.pone.0085957.g003 %2 https://ndownloader.figshare.com/files/1348925 %K Biochemistry %K proteins %K Transmembrane transport proteins %K Molecular cell biology %K Cellular structures %K Subcellular organelles %K Signal transduction %K Signaling cascades %K cAMP signaling cascade %K neuroscience %K Molecular neuroscience %K Signaling pathways %K Neurobiology of disease and regeneration %K mct1 %K patterns %K cells %K transfected %K deletion %K mcherry-mct1 %X

A. Protein motifs in the C and N termini of Mct1 that could be involved in controlling its localization to vesicles include (in red); type 1 and 4 WW ligands, an AP2 clathrin interaction site, a PDZ ligand, a hydrophobic N terminus, a charged C terminus (+ and −), lysine residues (shown in green), and numerous phosphorylation sites (PO4). B. Confocal micrographs showed a similar appearance of Mct1 vesicles among cells expressing FL, XC, and XN mCherry-Mct1. C. An epi-fluorescence micrograph of an RBE4 cell expressing the C-terminus of Mct1 with mCherry fused to its amino terminus.

%I PLOS ONE