%0 Figure %A Roberson, Elisha %A Mesa, Rosana %D 2019 %T NCSTN antibody validation - actin antibody in HEK293 knockout line after PNGase treatment %U https://figshare.com/articles/figure/NCSTN_antibody_validation_-_actin_antibody_in_HEK293_knockout_line_after_PNGase_treatment/8952983 %R 10.6084/m9.figshare.8952983.v1 %2 https://ndownloader.figshare.com/files/16369784 %K HEK293 %K nicastrin %K NCSTN knockout %K antibody %K antibody validation %K Cell Biology %X This is the original TIF image of an antibody blot stored in a scaleable vector graphic file. The tif has a dotted line approximately outlining the location of cropping for the paper figure.
The protein source was from HEK293 cells with a CRISPR/Cas9 knockout of nicastrin and wildtype HEK293 cells from the ATCC. One lane has PNGase treatment. The bands are from nicastrin antibody #AB6276. %I figshare