Zhang, Yuhong Xu, Xiaolu Zhou, Xiaojin Chen, Rumei Yang, Peilong Meng, Qingchang Meng, Kun Luo, Huiying Yuan, Jianhua Yao, Bin Zhang, Wei PCR analysis and southern blot analysis of the transgenic maize. <p><b>A</b> PCR detection of the gene <i>bgl7Am</i> in the genomic DNA of transgenic plant leaves of generation BC1. Lane 1, the plasmid PHP20754-<i>bgl7Am</i> as positive control; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>B</b> PCR detection of the gene <i>actin</i>. Lane 1, the plasmid PHP20754-<i>bgl7Am</i>; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>C</b> Southern blot analysis of <i>bgl7Am</i> in transgenic plants. The <i>Eco</i>RI and <i>Hind</i>III-digested genomic DNA was hybridized with the <i>bgl7Am</i> probe. Lane 1, the DIG-labeled molecular weight markers; lane 2–4, transgenic plants digested by <i>Eco</i>RI (arrowhead indicate the positive bands); lane 5, non-transgenic Zheng58 digested by <i>Eco</i>RI; lane 6, non-transgenic Zheng58 digested by <i>Hind</i>III; lane 7–9, transgenic plants digested by <i>Hind</i>III (arrowhead indicate the positive band); lane 10, PCR fragment of the <i>bgl7Am</i> as a positive control (arrowhead).</p> Agricultural biotechnology;Genetically modified organisms;crops;cereals;maize;Biochemistry;enzymes;Enzyme classes;glucanases;hydrolases;biocatalysis;biotechnology;Plant biotechnology;Bioengineering;Plant science;Transgenic plants;blot;transgenic 2013-12-31
    https://plos.figshare.com/articles/figure/_PCR_analysis_and_southern_blot_analysis_of_the_transgenic_maize_/892149
10.1371/journal.pone.0081993.g002