10.1371/journal.pone.0081993.g002 Yuhong Zhang Yuhong Zhang Xiaolu Xu Xiaolu Xu Xiaojin Zhou Xiaojin Zhou Rumei Chen Rumei Chen Peilong Yang Peilong Yang Qingchang Meng Qingchang Meng Kun Meng Kun Meng Huiying Luo Huiying Luo Jianhua Yuan Jianhua Yuan Bin Yao Bin Yao Wei Zhang Wei Zhang PCR analysis and southern blot analysis of the transgenic maize. Public Library of Science 2013 Agricultural biotechnology Genetically modified organisms crops cereals maize Biochemistry enzymes Enzyme classes glucanases hydrolases biocatalysis biotechnology Plant biotechnology Bioengineering Plant science Transgenic plants blot transgenic 2013-12-31 06:19:13 Figure https://plos.figshare.com/articles/figure/_PCR_analysis_and_southern_blot_analysis_of_the_transgenic_maize_/892149 <p><b>A</b> PCR detection of the gene <i>bgl7Am</i> in the genomic DNA of transgenic plant leaves of generation BC1. Lane 1, the plasmid PHP20754-<i>bgl7Am</i> as positive control; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>B</b> PCR detection of the gene <i>actin</i>. Lane 1, the plasmid PHP20754-<i>bgl7Am</i>; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>C</b> Southern blot analysis of <i>bgl7Am</i> in transgenic plants. The <i>Eco</i>RI and <i>Hind</i>III-digested genomic DNA was hybridized with the <i>bgl7Am</i> probe. Lane 1, the DIG-labeled molecular weight markers; lane 2–4, transgenic plants digested by <i>Eco</i>RI (arrowhead indicate the positive bands); lane 5, non-transgenic Zheng58 digested by <i>Eco</i>RI; lane 6, non-transgenic Zheng58 digested by <i>Hind</i>III; lane 7–9, transgenic plants digested by <i>Hind</i>III (arrowhead indicate the positive band); lane 10, PCR fragment of the <i>bgl7Am</i> as a positive control (arrowhead).</p>