10.1371/journal.pone.0081993.g002
Yuhong Zhang
Yuhong
Zhang
Xiaolu Xu
Xiaolu
Xu
Xiaojin Zhou
Xiaojin
Zhou
Rumei Chen
Rumei
Chen
Peilong Yang
Peilong
Yang
Qingchang Meng
Qingchang
Meng
Kun Meng
Kun
Meng
Huiying Luo
Huiying
Luo
Jianhua Yuan
Jianhua
Yuan
Bin Yao
Bin
Yao
Wei Zhang
Wei
Zhang
PCR analysis and southern blot analysis of the transgenic maize.
Public Library of Science
2013
Agricultural biotechnology
Genetically modified organisms
crops
cereals
maize
Biochemistry
enzymes
Enzyme classes
glucanases
hydrolases
biocatalysis
biotechnology
Plant biotechnology
Bioengineering
Plant science
Transgenic plants
blot
transgenic
2013-12-31 06:19:13
Figure
https://plos.figshare.com/articles/figure/_PCR_analysis_and_southern_blot_analysis_of_the_transgenic_maize_/892149
<p><b>A</b> PCR detection of the gene <i>bgl7Am</i> in the genomic DNA of transgenic plant leaves of generation BC1. Lane 1, the plasmid PHP20754-<i>bgl7Am</i> as positive control; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>B</b> PCR detection of the gene <i>actin</i>. Lane 1, the plasmid PHP20754-<i>bgl7Am</i>; lane 2–9, the transgenic plants; lane 10, the non-transgenic Zheng58. <b>C</b> Southern blot analysis of <i>bgl7Am</i> in transgenic plants. The <i>Eco</i>RI and <i>Hind</i>III-digested genomic DNA was hybridized with the <i>bgl7Am</i> probe. Lane 1, the DIG-labeled molecular weight markers; lane 2–4, transgenic plants digested by <i>Eco</i>RI (arrowhead indicate the positive bands); lane 5, non-transgenic Zheng58 digested by <i>Eco</i>RI; lane 6, non-transgenic Zheng58 digested by <i>Hind</i>III; lane 7–9, transgenic plants digested by <i>Hind</i>III (arrowhead indicate the positive band); lane 10, PCR fragment of the <i>bgl7Am</i> as a positive control (arrowhead).</p>