Inhibition of the ATPase activity of full-length NS3 by the compounds 9b and 9c. C. B. da CostaEmmerson AmorimRaquel C. da SilvaFernando R. RochaDavid P. PapaMichelle B. de ArrudaLuciana Mohana-BorgesRonaldo FerreiraVitor F. TanuriAmilcar J. da CostaLuciana FerreiraSabrina B. 2013 <p>Increasing concentrations of naphthoquinones 9b and 9c were tested for their activity against ATPase site. The reaction was performed in buffer containing 40 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM KCl, and 5 mM MgCl<sub>2</sub>. The compounds were pre-incubated with 600nM NS3FL for 10 min at 30 °C followed by the addition of 1 mM ATP. Each point represents the average of three independent replicates in different concentrations of the compounds.</p>