Dose-response curves and citotoxicity of 1,4-pyran naphthoquinones 9b and 9c in DENV-2-infected Vero cells.
Emmerson C. B. da Costa
Raquel Amorim
Fernando C. da Silva
David R. Rocha
Michelle P. Papa
Luciana B. de Arruda
Ronaldo Mohana-Borges
Vitor F. Ferreira
Amilcar Tanuri
Luciana J. da Costa
Sabrina B. Ferreira
10.1371/journal.pone.0082504.g004
https://plos.figshare.com/articles/figure/_Dose_response_curves_and_citotoxicity_of_1_4_pyran_naphthoquinones_9b_and_9c_in_DENV_2_infected_Vero_cells_/883658
<p>Vero cells were infected with DENV-2 at an m.o.i of 0.1 in the presence of increasing concentrations of the 1,4-pyran naphthoquinones 9b and 9c. 72 hours after infection the cell-free supernatants were harvested and processed for the quantification of produced viral progeny by qPCR. (A) The percentage of inhibition was calculated using the ΔΔCT values. (B) The concentration of each compound that inhibit 50% of the viral replication (IC<sub>50</sub>) was determined by a Hill’s regression curve after logarithmic interpolation of the data using the GraphPad 6 software. (C) The potential cytotoxic effect of each compound was evaluated in uninfected cells by the vital neutral red staying and represented as percentage of the untreated control. Data represent mean values ± standard deviations (SD) for three independent experiments.</p>
2013-12-20 03:35:35
curves
citotoxicity
naphthoquinones
9b
9c
denv-2-infected
vero