Migration of mt-SOD1-Dendra and mt-SOD1<sup>G93A</sup>-Dendra in normal muscle fibers.
Guo Luo
Jianxun Yi
Changling Ma
Yajuan Xiao
Frank Yi
Tian Yu
Jingsong Zhou
10.1371/journal.pone.0082112.g005
https://plos.figshare.com/articles/figure/_Migration_of_mt_SOD1_Dendra_and_mt_SOD1_G93A_Dendra_in_normal_muscle_fibers_/871136
<p>Representative images of muscle fibers expressing mt-SOD1-Dendra (<b>A</b>) or mt-SOD1<sup>G93A</sup>-Dendra (<b>B</b>). Before photoactivation Dendra was a green fluorescent protein (green images in left panels). A small region of the fiber was photoactivated. The photoactivation region in the fiber was marked with the box (white dashed line). After the photoactivation Dendra was converted to a red fluorescent protein (red images in right planes). (<b>C</b>) Migration of mt-SOD1-Dendra in the presence of 200 nM FCCP. Bar: 10 µm. (<b>D</b>) The migration time course of the red fluorescent protein. Solid lines are the linear regression of the data after 2 minutes of the photoactivation. Dashed lines are the linear regression of the data in the first 2 minutes, assuming the migration step is zero at time zero. The migration rates are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082112#pone-0082112-t001" target="_blank">Table <b>1</b></a>. (P<0.01).</p>
2013-12-06 03:31:32
mt-sod1-dendra