10.1371/journal.pone.0081063.g002 Subrata Biswas Subrata Biswas Deborah A. DiSilvestre Deborah A. DiSilvestre Peihong Dong Peihong Dong Gordon F. Tomaselli Gordon F. Tomaselli Current decay of myotonia mutant channels. Public Library of Science 2013 decay myotonia mutant 2013-12-06 03:28:41 Figure https://plos.figshare.com/articles/figure/_Current_decay_of_myotonia_mutant_channels_/871134 <p>The time constant of decay is altered in hNa<sub>V</sub>1.4<sub>F1705I</sub> compared to wild type hNa<sub>V</sub>1.4 channels. (<b>A</b>) Superimposed normalized currents through wild type and hNa<sub>V</sub>1.4<sub>F1705I</sub> channels at test pulses of −30, −20, and −10 mV exhibiting slowing of current decay in the mutant channel. (<b>B</b>) The time constants of current decay of hNa<sub>V</sub>1.4<sub>F1705I</sub> channels are significantly different compared to wild type in the absence (p<0.05) or presence of 0.5 µM of Ca<sup>2+</sup> (p<0.001). The inset is an expanded view −20 mV to 0 mV. (<b>C</b>) Raw current traces of hNa<sub>V</sub>1.4<sub>F1705I</sub> channels at RT and at 37°C. Currents were measured at −20 mV, in the same cell at different temperatures with 0.5 µM of Ca<sup>2+</sup> in the pipette. (<b>D</b>) Plot of the time constants of current decay of wild type and hNa<sub>V</sub>1.4<sub>F1705I</sub> channels at RT and at 37°C in 0.5 µM of Ca<sup>2+</sup>. Paired measurements were made at RT and at 37°C. The current decay of hNa<sub>V</sub>1.4<sub>F1705I</sub> is significantly different at 37°C compared to RT (p<0.05). (<b>E</b>) Plot of the steady-state inactivation of hNa<sub>V</sub>1.4<sub>F1705I</sub> channels at 37°C in 0.5 µM of Ca<sup>2+</sup>. For comparison the steady-state inactivation of wild type and hNa<sub>V</sub>1.4<sub>F1705I</sub> from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081063#pone-0081063-g001" target="_blank">Figure 1E</a> are re-plotted. The symbols are the same in plots B, D and E.</p>