%0 Figure %A J. Kerkhoven, Eduard %A Achcar, Fiona %A Alibu, Vincent P. %A J. Burchmore, Richard %A Gilbert, Ian H. %A Trybiło, Maciej %A N. Driessen, Nicole %A Gilbert, David %A Breitling, Rainer %A M. Bakker, Barbara %A Barrett, Michael P. %D 2013 %T Simulations of 6PGDH inhibition and 6-PG accumulation. %U https://plos.figshare.com/articles/figure/Simulations_of_6PGDH_inhibition_and_6_PG_accumulation_/869004 %R 10.1371/journal.pcbi.1003371.g005 %2 https://ndownloader.figshare.com/files/1304051 %K 6pgdh %K inhibition %K 6-pg %X

(A–B) The effects of inhibition of 6PGDH on 6-PG concentrations and metabolic fluxes were simulated by reducing Vmax,6PGDH in model C and D at high oxidative stress (kTOX = 200 µl·min−1·mg protein−1). Simulations at low oxidative stress (kTOX = 2 µl·min−1·mg protein−1) are shown in Figure S6. ATP production flux as steady-state flux through PFK is indicated in red, while trypanothione reductase steady-state flux is indicated in yellow, both plotted on the left y-axis. Steady-state concentration of cytosolic (blue) and glycosomal (green) 6-phosphogluconate are plotted on the right y-axis. Shaded areas indicate interquartile ranges. (C) Steady-state flux through glycolysis as a function of the glycosomal 6-PG concentration in model A. A glycosomal 6-PG concentration of around 500 mM reduces the glycolytic flux by 50%.

%I PLOS Computational Biology