TY - DATA T1 - ATP:ADP antiporter mimics turbo-state. PY - 2013/12/05 AU - Eduard J. Kerkhoven AU - Fiona Achcar AU - Vincent P. Alibu AU - Richard J. Burchmore AU - Ian H. Gilbert AU - Maciej Trybiło AU - Nicole N. Driessen AU - David Gilbert AU - Rainer Breitling AU - Barbara M. Bakker AU - Michael P. Barrett UR - https://plos.figshare.com/articles/figure/ATP_ADP_antiporter_mimics_turbo_state_/869002 DO - 10.1371/journal.pcbi.1003371.g003 L4 - https://ndownloader.figshare.com/files/1304049 KW - antiporter KW - mimics N2 - (A) Overview of the models used in this figure. Model A and D are from Figure 1, model A–glyc is model A without glycosomal localization, as described in [31], model A+AAT is model A with an ATP:ADP antiporter. (B–C) Steady-state concentrations of glycosomal Glc-6-P and Fru-1,6-BP are depicted in the various models. (D) Increasing the activity of the ATP:ADP antiporter (Vmax,ATP:ADP antiporter) in model D leads to a high risk of accumulation of hexose phosphates. The green line indicates the concentration of Fru-1,6-BP in the original model of glycolysis (17.2 mM, panel C, model A). Glce in this simulation is 25 mM. (E) Time course simulation of model D at 25 mM Glce and various values for the Vmax,ATP:ADP antiporter parameter. Plotted is the concentration of glycosomal phosphates (ΣP similar as in Figure 2, moiety 5 in Table 2). ATP:ADP antiporter activity values below 1 nmol·min−1·mg protein−1 result in depletion of glycosomal phosphates (cf. Figure 2). kTOX = 2 µl·min−1·mg protein−1 in all models. Solid lines indicate medians, shaded areas and error bars show interquartile ranges, as derived from the uncertainty modeling. ER -