10.1371/journal.pntd.0002548
Jing-ying Xiao
Jing-ying
Xiao
Ji-Yun Lee
Ji-Yun
Lee
Shinji Tokuhiro
Shinji
Tokuhiro
Mitsuru Nagataki
Mitsuru
Nagataki
Blanca R. Jarilla
Blanca
R. Jarilla
Haruka Nomura
Haruka
Nomura
Tae Im Kim
Tae
Im Kim
Sung-Jong Hong
Sung-Jong
Hong
Takeshi Agatsuma
Takeshi
Agatsuma
Molecular Cloning and Characterization of Taurocyamine Kinase from <i>Clonorchis sinensis</i>: A Candidate Chemotherapeutic Target
Public Library of Science
2013
molecular
cloning
characterization
taurocyamine
kinase
chemotherapeutic
2013-11-21 04:40:34
Dataset
https://plos.figshare.com/articles/dataset/Molecular_Cloning_and_Characterization_of_Taurocyamine_Kinase_from_Clonorchis_sinensis_A_Candidate_Chemotherapeutic_Target/859974
<div><p>Background</p><p>Adult <i>Clonorchis sinensis</i> lives in the bile duct and causes endemic clonorchiasis in East Asian countries. Phosphagen kinases (PK) constitute a highly conserved family of enzymes, which play a role in ATP buffering in cells, and are potential targets for chemotherapeutic agents, since variants of PK are found only in invertebrate animals, including helminthic parasites. This work is conducted to characterize a PK from <i>C. sinensis</i> and to address further investigation for future drug development.</p><p>Methology/Principal findings</p><p>A cDNA clone encoding a putative polypeptide of 717 amino acids was retrieved from a <i>C. sinensis</i> transcriptome. This polypeptide was homologous to taurocyamine kinase (TK) of the invertebrate animals and consisted of two contiguous domains. <i>C. sinensis</i> TK (CsTK) gene was reported and found consist of 13 exons intercalated with 12 introns. This suggested an evolutionary pathway originating from an arginine kinase gene group, and distinguished annelid TK from the general CK phylogenetic group. CsTK was found not to have a homologous counterpart in sequences analysis of its mammalian hosts from public databases. Individual domains of CsTK, as well as the whole two-domain enzyme, showed enzymatic activity and specificity toward taurocyamine substrate. Of the CsTK residues, R58, I60 and Y84 of domain 1, and H60, I63 and Y87 of domain 2 were found to participate in binding taurocyamine. CsTK expression was distributed in locomotive and reproductive organs of adult <i>C. sinensis</i>. Developmentally, CsTK was stably expressed in both the adult and metacercariae stages. Recombinant CsTK protein was found to have low sensitivity and specificity toward <i>C. sinensis</i> and platyhelminth-infected human sera on ELISA.</p><p>Conclusion</p><p>CsTK is a promising anti-<i>C. sinensis</i> drug target since the enzyme is found only in the <i>C. sinensis</i> and has a substrate specificity for taurocyamine, which is different from its mammalian counterpart, creatine.</p></div>