Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes NelsonChas BuckleyCharlotte MullinsJohn J DenvirMartin A TaylorJonathan M 2019 <div>How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.</div><div><br></div><div>Presented at <a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/browse/SPIE-Photonics-West/SPIE-BiOS/2018">SPIE BiOS</a>, 2018, San Francisco, California, United States. Paper 104991F.</div><div><br></div><div>Yellow frames indicate animations/videos.<br></div>