%0 Figure %A A. Onasoga-Jarvis, Abimbola %A Leiderman, Karin %A L. Fogelson, Aaron %A Wang, Michael %A J. Manco-Johnson, Marilyn %A A. Di Paola, Jorge %A Neeves, Keith B. %D 2013 %T Thrombi formed under flow on collagen-TF surfaces from individuals with FVIII deficiencies. %U https://plos.figshare.com/articles/figure/_Thrombi_formed_under_flow_on_collagen_TF_surfaces_from_individuals_with_FVIII_deficiencies_/848857 %R 10.1371/journal.pone.0078732.g001 %2 https://ndownloader.figshare.com/files/1278506 %K formed %K collagen-tf %K surfaces %K individuals %K fviii %X

Recalcified whole blood was perfused over glass slides coated with 2.3/cm2 and type 1 fibrillar collagen at 100 s−1 for 5 min. Representative images of platelets (blue, anti-CD41) and fibrin(ogen) (green, Alexa488-fibrinogen) accumulation for a normal control (A) and hemophilia samples with plasma FVIII levels of 11.1% (B), 3.1% (C), and 0.4% (D) at 5 min. Scale bar = 25 µm. Scanning electron micrographs of thrombi from the same individuals; (E) 100% 11.1% (F), 3.1% (G), and 0.4% (H). Platelet aggregates are immersed in a fibrin mesh for the control (E) and form a starburst like pattern for mild hemophilia samples (F). The fibers on the surface in (G) and (H) are collagen fibers. Scale bar = 25 µm.

%I PLOS ONE