10.1371/journal.pone.0079518.g004 Yu-Lin Hsu Yu-Lin Hsu Shao-Fu Shi Shao-Fu Shi Wan-Lin Wu Wan-Lin Wu Ling-Jun Ho Ling-Jun Ho Jenn-Haung Lai Jenn-Haung Lai Synergistic pro-apoptotic effects of both knockdown of IFIT3 and DV infection. Public Library of Science 2013 pro-apoptotic knockdown ifit3 dv 2013-11-04 03:44:20 Figure https://plos.figshare.com/articles/figure/_Synergistic_pro_apoptotic_effects_of_both_knockdown_of_IFIT3_and_DV_infection_/841378 <p>A549 cells transfected with control siRNA (si-Ctl) or IFIT3 siRNA (siIFIT3) for 24 h were infected with mock or DV at M.O.I. = 0.5 or 5 for an additional 48 h. Cell viability was determined by MTT assays. Mock-infected cells treated with control siRNA transfection were taken as 100%, and OD values from individual conditions were normalized by the value of the control (% of control; A). DNA content was determined by sub-G1 analysis (left panel in B), and the synergistic effects were calculated (right panel in B). Determination and visualization of cell apoptosis were also performed by TUNEL assays using flow cytometry and immunofluorescent staining (C). Data show representative results and analysis pooled from at least 3 independent experiments. The analysis was performed by ANOVA as described in Materials and Methods. *P < 0.05, **P < 0.01, ***P < 0.001. Ctl stands for control.</p>