Stopped-flow analysis of SR-1 RW and GroEL R231W: dependence on prebound MDH and GroES.
Toshifumi Mizuta
Kasumi Ando
Tatsuya Uemura
Yasushi Kawata
Tomohiro Mizobata
10.1371/journal.pone.0078135.g007
https://plos.figshare.com/articles/dataset/_Stopped_flow_analysis_of_SR_1_RW_and_GroEL_R231W_dependence_on_prebound_MDH_and_GroES_/837357
<p>A. (<i>upper panels</i>) Analysis of SR-1-RW initiated by addition of ATP only (<i>left</i>, “-GroES”) or ATP and equimolar GroES (<i>right</i>, “+GroES”). <i>Black traces</i> indicate GroEL SR-1 RW only, <i>gray traces</i> indicate SR-1 RW with prebound MDH. B. (<i>lower panels</i>) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.</p>
2013-10-30 19:52:53
Dynamic Process
chaperonin function
GroEL apical domain
GroEL D 398A variants
apical domain movement
GroEL rings
GroEL SR
ATP binding
circularly permuted GroEL
polypeptide ends
GroES binding
Escherichia coli GroEL Kinetic analyses
CP
GroEL mutants
Helix M
ATP binding site