Stopped-flow analysis of SR-1 RW and GroEL R231W: dependence on prebound MDH and GroES. Toshifumi Mizuta Kasumi Ando Tatsuya Uemura Yasushi Kawata Tomohiro Mizobata 10.1371/journal.pone.0078135.g007 https://plos.figshare.com/articles/dataset/_Stopped_flow_analysis_of_SR_1_RW_and_GroEL_R231W_dependence_on_prebound_MDH_and_GroES_/837357 <p>A. (<i>upper panels</i>) Analysis of SR-1-RW initiated by addition of ATP only (<i>left</i>, “-GroES”) or ATP and equimolar GroES (<i>right</i>, “+GroES”). <i>Black traces</i> indicate GroEL SR-1 RW only, <i>gray traces</i> indicate SR-1 RW with prebound MDH. B. (<i>lower panels</i>) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.</p> 2013-10-30 19:52:53 Dynamic Process chaperonin function GroEL apical domain GroEL D 398A variants apical domain movement GroEL rings GroEL SR ATP binding circularly permuted GroEL polypeptide ends GroES binding Escherichia coli GroEL Kinetic analyses CP GroEL mutants Helix M ATP binding site