Morii, Kenichi Yamasaki, Satoshi Doi, Shigehiro Irifuku, Taisuke Sasaki, Kensuke Doi, Toshiki Nakashima, Ayumu Arihiro, Koji Masaki, Takao Summary of the results. <p>In HK-2 cells, oxidative stress induced pri-miR-200c and miR-200c, a miRNA that is complementary with the 3′-UTR of <i>KLOTHO</i> mRNA at two different sites. miR-200c binds to the <i>KLOTHO</i> mRNA 3′-UTR with Argonaute protein (AGO) to suppress the expression of KLOTHO by inhibiting translation. Transfection of a miR-200c mimic reduced KLOTHO expression without a reduction in <i>KLOTHO</i> mRNA levels and transfection of a miR-200c inhibitor maintained KLOTHO expression.</p> oxidative stress markers;KLOTHO mRNA levels;KLOTHO expression;upregulates microRNA -200c;miR -200c inhibitor;KLOTHO mRNA metabolism;upregulated miR -200c expression;oxidative stress-induced miR -200c;HK -2 cells;miR -200c;kidney biopsy specimens;H 2 O 2;UTR;h 2 O 2;oxidative stress KLOTHO deficiency 2019-06-14
    https://plos.figshare.com/articles/figure/Summary_of_the_results_/8279246
10.1371/journal.pone.0218468.g007