Summary of the results. Kenichi Morii Satoshi Yamasaki Shigehiro Doi Taisuke Irifuku Kensuke Sasaki Toshiki Doi Ayumu Nakashima Koji Arihiro Takao Masaki 10.1371/journal.pone.0218468.g007 https://plos.figshare.com/articles/figure/Summary_of_the_results_/8279246 <p>In HK-2 cells, oxidative stress induced pri-miR-200c and miR-200c, a miRNA that is complementary with the 3′-UTR of <i>KLOTHO</i> mRNA at two different sites. miR-200c binds to the <i>KLOTHO</i> mRNA 3′-UTR with Argonaute protein (AGO) to suppress the expression of KLOTHO by inhibiting translation. Transfection of a miR-200c mimic reduced KLOTHO expression without a reduction in <i>KLOTHO</i> mRNA levels and transfection of a miR-200c inhibitor maintained KLOTHO expression.</p> 2019-06-14 17:41:46 oxidative stress markers KLOTHO mRNA levels KLOTHO expression upregulates microRNA -200c miR -200c inhibitor KLOTHO mRNA metabolism upregulated miR -200c expression oxidative stress-induced miR -200c HK -2 cells miR -200c kidney biopsy specimens H 2 O 2 UTR h 2 O 2 oxidative stress KLOTHO deficiency