TY - DATA T1 - A green and bio-inspired process to afford durable anti-biofilm properties to stainless steel PY - 2012/08/01 AU - E. Faure AU - C. Vreuls AU - G. Zocchi AU - C. Van De Weerdt AU - J. Martial AU - C. Detrembleur AU - C. Falentin-Daudré AU - C. Jérôme AU - A.-S. Duwez UR - https://tandf.figshare.com/articles/journal_contribution/A_green_and_bio_inspired_process_to_afford_durable_anti_biofilm_properties_to_stainless_steel/825597 DO - 10.6084/m9.figshare.825597.v1 L4 - https://ndownloader.figshare.com/files/1240822 KW - bio-inspired KW - durable KW - anti-biofilm KW - stainless N2 - A bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and assembled from aqueous solutions onto SS surfaces to afford durable coatings. Trypsin, a commercially available broad spectrum serine protease, was grafted as the final active layer of the coating. Its proteolytic activity after long immersion periods was demonstrated against several substrata, viz. a synthetic molecule, N-α-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA), a protein, FTC-casein, and Gram-positive biofilm forming bacterium Staphylococcus epidermidis. ER -