Additional file 6: of Nalbuphine suppresses breast cancer stem-like properties and epithelial-mesenchymal transition via the AKT-NFκB signaling pathway YuJiachuan LuoYuanyuan WenQingping 2019 Figure S5. Nalbuphine inhibits breast cancer stem-like properties through the AKT-NFκB pathway. (A) MDA-MB-231 cells were treated with nalbuphine and/or IGF-1 for 48 h and levels of indicated proteins were determined by western blot (n = 3). (B) MDA-MB-231 cells transfected with shPTEN were treated with SC79 for 48 h and levels of indicated proteins were determined by western blot (n = 3). (DOCX 445 kb)