TY - DATA T1 - Flow cytometry data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice PY - 2013/10/17 AU - Aslam Mohammad AU - Yusuke Kishi AU - Takeshi Tsubata UR - https://f1000.figshare.com/articles/dataset/Flow_cytometry_data_of_spleen_cells_from_wild_type_3H9_transgenic_and_CD40L_3H9_double_transgenic_mice/806234 DO - 10.6084/m9.figshare.806234.v1 L4 - https://ndownloader.figshare.com/files/1264302 L4 - https://ndownloader.figshare.com/files/1264300 L4 - https://ndownloader.figshare.com/files/1264301 L4 - https://ndownloader.figshare.com/files/1264303 L4 - https://ndownloader.figshare.com/files/1264304 L4 - https://ndownloader.figshare.com/files/1264305 KW - cd40l KW - b cells KW - auto-immunity KW - clonal anergy KW - Immunology N2 - Data 1. Flow cytometry analysis of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.Spleen cells were obtained from wild type (A and D), 3H9-transgenic (B and E), and CD40L/3H9 double transgenic mice (C and F). Cells were stained with Alexa Fluor 647-conjugated (FL 8 channel) rat anti-mouse B220 and Pacific Blue-conjugated (FL 6 channel) anti-mouse Ig λ1(LS-136), and with (D-F) or without (A-C) FITC-conjugated (FL 1 channel) goat anti-mouse Ig λ chain. Lymphoid cells were gated by FSC/SSC dot plots and the B220+ cells were analyzed on a CyAn ADP flow cytometer (Beckman Coulter, USA). ER -