Confocal immunofluorescent analysis showing MICA/B staining. Yessica L. Allegretti Constanza Bondar Luciana Guzman Eduardo Cueto Rua Nestor Chopita Mercedes Fuertes Norberto W. Zwirner Fernando G. Chirdo 10.1371/journal.pone.0073658.g002 https://plos.figshare.com/articles/figure/_Confocal_immunofluorescent_analysis_showing_MICA_B_staining_/799657 <p><b>A.</b>- sample from an untreated CD pediatric patient with mild enteropathy showing the MICA/B expression (red) in enterocytes. SYTO® 13 (Green Fluorescent Nucleic Acid Stain) was used to stain nuclei. <b>B.</b>- MICA/B staining in an intestinal section from the same patient after two years on a gluten-free diet. <b>C.</b>- healthy non-celiac control patient. <b>D.</b> IC incubated in a section corresponding to sample A. (Magnification 63×). <b>E.</b>- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3<sup>−</sup> cells of a representative paediatric patient. <b>F.</b>- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3<sup>−</sup> cells of duodenal samples from adult CD patients.</p> 2013-09-13 02:30:46 immunofluorescent