10.1371/journal.pone.0073658.g002 Yessica L. Allegretti Yessica L. Allegretti Constanza Bondar Constanza Bondar Luciana Guzman Luciana Guzman Eduardo Cueto Rua Eduardo Cueto Rua Nestor Chopita Nestor Chopita Mercedes Fuertes Mercedes Fuertes Norberto W. Zwirner Norberto W. Zwirner Fernando G. Chirdo Fernando G. Chirdo Confocal immunofluorescent analysis showing MICA/B staining. Public Library of Science 2013 immunofluorescent 2013-09-13 02:30:46 Figure https://plos.figshare.com/articles/figure/_Confocal_immunofluorescent_analysis_showing_MICA_B_staining_/799657 <p><b>A.</b>- sample from an untreated CD pediatric patient with mild enteropathy showing the MICA/B expression (red) in enterocytes. SYTO® 13 (Green Fluorescent Nucleic Acid Stain) was used to stain nuclei. <b>B.</b>- MICA/B staining in an intestinal section from the same patient after two years on a gluten-free diet. <b>C.</b>- healthy non-celiac control patient. <b>D.</b> IC incubated in a section corresponding to sample A. (Magnification 63×). <b>E.</b>- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3<sup>−</sup> cells of a representative paediatric patient. <b>F.</b>- Flow cytometric analysis for surface and intracellular expression of MICA/B in epithelial CD3<sup>−</sup> cells of duodenal samples from adult CD patients.</p>