10.1371/journal.pone.0214803.g006
Takuma Tanabe
Takuma
Tanabe
Masayuki Yamaga
Masayuki
Yamaga
Makoto Kawamukai
Makoto
Kawamukai
Yasuhiro Matsuo
Yasuhiro
Matsuo
Overexpression of Mal3 (1–143) exhibits the TBZ-tolerance in the wild type strain.
Public Library of Science
2019
multi-copy suppressor
cAMP-dependent protein kinase Pka 1
mal 3 gene
89E
EB 1 orthologs
microtubule-destabilization drug TBZ
fission yeast pka 1
Mal 3
TBZ-sensitive phenotype
chromosome mis-segregation
Mal 3 CH domain
Pka 1
high-affinity microtubule binding
pka 1 gene
chromosome segregation
strain
89R
Mal 3. Overexpression
2019-04-11 17:34:46
Figure
https://plos.figshare.com/articles/figure/Overexpression_of_Mal3_1_143_exhibits_the_TBZ-tolerance_in_the_wild_type_strain_/7983908
<p>(A) Wild type (PR109) strains harboring pREP3X (vector), pREP3X-mal3 (1–308; full-length), pREP3X-mal3 (1–143), or pREP3X-mal3 (135–308) were cultured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0214803#pone.0214803.g001" target="_blank">Fig 1C</a>. Cells were streaked on EMMU in the presence or absence of 40 μg/mL TBZ or 60 μg/mL TBZ. All plates were incubated for 5 days at 30˚C. (B) <i>S</i>. <i>cerevisiae</i> AH109 strain was co-transformed with pGBKT7-derived plasmid and pGAD424-derived plasmid and selected on SC lacking leucine and tryptophan (SC-LW). Transformed cells were cultured and the cell suppression was spotted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0214803#pone.0214803.g002" target="_blank">Fig 2B</a>. All plates were incubated for 3 days at 30˚C.</p>