Wu, Hao Wang, You Li, Wenhui Chen, Hui Du, Lei Liu, Dong Wang, Xiaohui Xu, Tao Liu, Lei Chen, Quan Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome <p>There is overwhelming evidence for an association between impaired mitochondrial function and metabolic syndrome. Mitophagy, a process that selectively removes damaged mitochondria <i>via</i> a specialized form of autophagy, is essential for mitochondrial quality control (mitochondrial QC) and metabolic homeostasis. We thus addressed the potential role of defective mitophagy in the pathogenesis of metabolic disorders. Mice lacking <i>Fundc1</i>, a newly characterized mitophagy receptor, develop more severe obesity and insulin resistance when fed a high-fat diet (HFD). Ablation of <i>Fundc1</i> results in defective mitophagy and impaired mitochondrial QC <i>in vitro</i> and in white adipose tissue (WAT). In addition, there is more pronounced WAT remodeling with more adipose tissue-associated macrophages infiltration, more M1 macrophage polarization and thus an elevated inflammatory response. Mechanistically, hyperactivation of MAPK/JNK leads to insulin insensitivity, which can be inhibited by knocking out <i>Mapk8</i>/<i>Jnk1</i> in <i>fundc1</i> KO mice. Our results demonstrate that dysregulated mitochondrial QC due to defective mitophagy receptor FUNDC1 links with metabolic disorders <i>via</i> MAPK signaling and inflammatory responses.</p> <p><b>Abbreviations</b>: ATMs: adipose tissue macrophages; BAT: brown adipose tissue; BMDMs: bone marrow-derived macrophages; GOT1/AST: glutamic-oxaloacetic transaminase 1, soluble; GPT/ALT: glutamic pyruvic transaminase, soluble; H&E staining: hematoxylin and eosin staining; HFD: high-fat diet; LIR: LC3-interacting region; mitochondrial QC: mitochondrial quality control; mito-ROS: mitochondrial ROS; mtDNA: mitochondrial DNA; RT-PCR: real-time-PCR; T2D: type 2 diabetes; WAT: white adipose tissue</p> FUNDC1;insulin resistance;MAPK;mitochondrial QC;mitophagy;obesity 2019-04-06
    https://tandf.figshare.com/articles/dataset/Deficiency_of_Mitophagy_Receptor_FUNDC1_Impairs_Mitochondrial_Quality_and_Aggravates_Dietary-Induced_Obesity_and_Metabolic_Syndrome/7879415
10.6084/m9.figshare.7879415.v2