%0 Figure %A J. Hiscox, Thomas %A F. Harrison, Paul %A Chakravorty, Anjana %A M. Choo, Jocelyn %A Ohtani, Kaori %A Shimizu, Tohru %A K. Cheung, Jackie %A I. Rood, Julian %D 2013 %T Sialidase and hyaluronidase production by the wild-type, mutant and complemented strains. %U https://plos.figshare.com/articles/figure/Sialidase_and_hyaluronidase_production_by_the_wild_type_mutant_and_complemented_strains_/787758 %R 10.1371/journal.pone.0073525.g002 %2 https://ndownloader.figshare.com/files/1189723 %K hyaluronidase %K mutant %K complemented %X

Quantitative assays were carried out to determine the relative amount of extracellular enzyme production by the wild-type, mutant and complemented strains at time of inoculation and 4 h and 8 h post-inoculation (corresponding to exponential and stationary growth phases, respectively). Sialidase assays were performed using culture supernatants from cultures grown in Todd-Hewitt broth (A). Hyaluronidase activity was determined using the cell lysates from cultures grown in TPYG (B). All results are given as the average of three biological replicates (± SEM) for sialidase assays and six biological replicates for hyaluronidase assays; the asterisk (*) denotes a statistically significant difference (p≤0.05) as determined by the student’s t-test.

%I PLOS ONE