%0 Figure %A Boss, Lidia %A Labudda, Łukasz %A Węgrzyn, Grzegorz %A Hayes, Finbarr %A Kędzierska, Barbara %D 2013 %T Figure S1 - The Axe-Txe Complex of Enterococcus faecium Presents a Multilayered Mode of Toxin-Antitoxin Gene Expression Regulation %U https://plos.figshare.com/articles/figure/The_Axe_Txe_Complex_of_Enterococcus_faecium_Presents_a_Multilayered_Mode_of_Toxin_Antitoxin_Gene_Expression_Regulation/787241 %R 10.1371/journal.pone.0073569.s001 %2 https://ndownloader.figshare.com/files/1188994 %K axe-txe %K presents %K multilayered %K toxin-antitoxin %X

Neither Axe-Txe proteins nor other proteins in the E. coli extract bound detectably to a fragment bearing the wild-type paxe promoter. A 126 bp 5’ biotinylated fragment that includes paxe was subjected to EMSA. DNA samples were incubated with the different crude extracts concentrations of E. coli BL21(DE3) harbouring pET22at_axe-txe plasmid (left to right): 0, 1.25, 2.5, 5, 10, 12.5 and 25 µg/ml for 20 min at 220C and analyzed by a native 5% PAGE. Reactions were processed as outlined in Materials and Methods.

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%I PLOS ONE