TY - DATA T1 - Human CCA-adding enzyme is able to repair damaged CCA ends of tRNAs. PY - 2013/08/29 AU - Andreas Czech AU - Sandra Wende AU - Mario Mörl AU - Tao Pan AU - Zoya Ignatova UR - https://plos.figshare.com/articles/figure/_Human_CCA_adding_enzyme_is_able_to_repair_damaged_CCA_ends_of_tRNAs_/784930 DO - 10.1371/journal.pgen.1003767.g003 L4 - https://ndownloader.figshare.com/files/1185968 KW - cca-adding KW - enzyme KW - damaged KW - cca KW - ends N2 - (A) Total HeLa tRNAs and (B) internally radioactively labeled yeast tRNAPhe were incubated successively with angiogenin and human CCA-adding enzyme. Subsequently to the angiogenin treatment (4 h), T4 polynucleotide kinase (PNK) (45 min) was added which converts the 2′,3′-cyclophosphate ends [42] generated by the angiogenin cleavage to free 3′OH. After purification tRNAs were subjected to treatment with the CCA-adding enzyme (30 min). The 3′-CCA end integrity of the HeLa tRNAs was determined with the fluorescent oligonucleotide (Figure 1A, schematic inset). tRNAPhe lacking the terminal 3′-adenosine (tRNAPheCC) served as a control. The numbers on the left denote the DNA ladder in nt. ER -