Figure 2. Deletion of Arrb1 attenuates the VEGF-C/VEGFR3 signaling in mice exposed to hypoxia. Zhiyuan Ma 10.6084/m9.figshare.7823666.v1 https://figshare.com/articles/figure/Figure_2_Deletion_of_Arrb1_attenuates_the_VEGF-C_VEGFR3_signaling_in_mice_exposed_to_hypoxia_/7823666 <p>(<b>a</b>) Heat map showing differential expressed genes at false discovery rate (FDR, < 0.01) between WT and <i>Arrb1</i> KO mice subjected to hypoxia (n = 3 mice per group). <i>Vegfc</i> expression is pointed by a red arrow. (<b>b</b>) Decreased mRNA expression by RNA-seq of <i>Vegfc</i>, <i>Lyve1 </i>and <i>vwf</i> in the lung of <i>Arrb1</i> KO compared to WT mice exposed to hypoxia. Statistical analysis was performed by CuffDiff. (<b>c</b>) RT-qPCR mRNA expression of <i>Vegfc</i>, <i>Lyve1</i> and <i>Vegfb</i> in the lungs of WT, <i>Arrb</i> KO, and <i>Arrb2</i> KO mice subjected to normoxia or hypoxia (n = 3 mice per group). (<b>d</b>) Protein expression in lung lystates from WT, <i>Arrb1</i> KO, and <i>Arrb2</i> KO mice (n = 3 mice per group). Quantification and normalization to WT exposed to normoxia demonstrates (<b>e</b>) a significant decrease in VEGFR3 and Akt phosphorylation in <i>Arrb1</i> KO mice. Statistical analysis was performed by one-way ANOVA and Tukey's multiple comparison test. (<b>f</b>) Loss of VEGFR3 in IPAH lung sections stained for LYVE1 (green), VEGFR3 (red), and nuclei (Hoechst; blue). Scale bar = 50 μm. (g) Inhibiting VEGFR3 signaling by the VEGFR3 inhibitor MAZ51 exacerbates PAH in WT mice (n = 8 mice per group), but not <i>Arrb1</i> KO mice (n = 9 mice per group). Statistical analysis was performed by two-tailed unpaired t test. *, p < 0.05; ***, p < 0.001.</p> 2019-03-15 22:27:35 vegfr3 beta-arrestin Cardiology Cell Biology