10.6084/m9.figshare.7814432.v1 Luis Javier Galindo Luis Javier Galindo Yana Eglit Yana Eglit Guifré Torruella Guifré Torruella Figure S1. Lithocolla globosa figshare 2019 Lithocolla globosa Lithocolla Evolutionary Biology 2019-03-07 18:06:10 Journal contribution https://figshare.com/articles/journal_contribution/Figure_S1_Lithocolla_globosa/7814432 <div>Figure S1. Lithocolla globosa SnP. A-B) Agglutinated cells from enrichment with sediment still</div><div>present. Surface view (B) showing attached mineral particles. C-O) Cells from culture, fed on</div><div>Navicula (C-G), Isochrysis (H,L-O) and Phaeodactylum (I-K). C-D) General view of same cell.</div><div>Note nucleus (in C) and ingested diatoms (in D), orange globules (D inset, bracket) and layer</div><div>(D inset, arrowhead). E) General view of cell. Note nucleus, orange globules and greenish</div><div>digestive vacuoles. F-H) Examples of intact filopodia. Note that filopodia degrade rapidly under</div><div>exposure to bright light. I-J) Individuals with 2 (I) and 3 (J) nuclei, relatively commonly observed</div><div>in culture. K) 'Re-agglutinated' cell one day following addition of chalk dust (as fine calcium</div><div>carbonate mineral inclusions) to culture. Note mineral particles inside and on the surface of the</div><div>cell, resembling the agglutinated cells from enrichment in (A-B). L-O) Pseudoseries of different</div><div>individuals showing mineral inclusions found in cells (arrowheads), 20min (L), 23min (M), 47min</div><div>(N), 60min (O) following addition of chalk dust to mineral-particle-deprived culture, indicating that</div><div>mineral particles are ingested by the cell. Differential interference contrast, scalebars are 20μm</div><div>(A-K, all images at same scale), 10μm (L-O).</div>