10.1371/journal.pone.0072675.g001 Günther Zick Günther Zick Gunnar Elke Gunnar Elke Tobias Becher Tobias Becher Dirk Schädler Dirk Schädler Sven Pulletz Sven Pulletz Sandra Freitag-Wolf Sandra Freitag-Wolf Norbert Weiler Norbert Weiler Inéz Frerichs Inéz Frerichs Study flowchart. Public Library of Science 2013 Anatomy and physiology Respiratory system Model organisms Animal models anesthesiology Anesthesiology monitoring Critical care and emergency medicine Respiratory failure Ventilatory support Pulmonology radiology Diagnostic radiology 2013-08-22 02:25:22 Figure https://plos.figshare.com/articles/figure/_Study_flowchart_/780434 <p>Ten animals were studied during volume-controlled ventilation during ventilation with 5 ml/kg (baseline) and 10 ml/kg tidal volume (V<sub>T</sub>) (measurement time point 1) in the normal lung (NL) as well as after induction of acute lung injury (ALI) (time point 2) at inspired fractions of oxygen of 0.5 and 1.0, respectively. Then a constant low-flow inflation maneuver (pressure-volume (PV) maneuver) was performed and positive end-expiratory pressure (PEEP) was set 2 cm H<sub>2</sub>O above the lower inflection point (LIP) identified in the PV curve. Using a crossover design, further measurements were performed 5 and 30 min after ventilation with low V<sub>T</sub> and active interventional lung assist (ILA) and after another 5 and 30 min with high V<sub>T</sub> and inactive ILA (no ILA) (time points 3–6). Five animals were randomly ventilated in the reversed chronological order. Ventilator settings of V<sub>T</sub> and PEEP at each measurement time point are shown in the lower part of the Figure. *, time elapsed after the change in ventilator and ILA settings.</p>