Effect of LTB<sub>4</sub>- or cysLT-receptor antagonists on macrophage phagocytosis and fungicidal activity against <i>P. brasiliensis</i>.
Patrícia Campi Santos
Daniel Assis Santos
Lucas Secchim Ribeiro
Caio Tavares Fagundes
Talles Prosperi de Paula
Thiago Vinícius Avila
Ludmila de Matos Baltazar
Mila Moreira Madeira
Rosana de Carvalho Cruz
Ana Carolina Fialho Dias
Fabiana Simão Machado
Mauro Martins Teixeira
Patrícia Silva Cisalpino
Danielle G. Souza
10.1371/journal.pntd.0002390.g006
https://plos.figshare.com/articles/figure/_Effect_of_LTB_4_or_cysLT_receptor_antagonists_on_macrophage_phagocytosis_and_fungicidal_activity_against_P_brasiliensis_/780329
<p>Peritoneal WT macrophages were treated with vehicle, LTB<sub>4</sub> receptor antagonist CP-105,696 (CP) (1 mM) or cysteinyl-LT receptor antagonist montelukast (MK) (10 mM) for 30 min before the addition of <i>P. brasiliensis</i> yeasts. Phagocytic index (A) and intracellular killing (B) were calculated 24 h after incubation. Killing assay was normalized according initial ingestion (PI) and presented as <i>P. brasiliensis</i> survival. The supernatants were harvested and nitrite concentrations (C) were measured. (D) ROS concentrations were determined by mean fluorescence intensity (DHR-123) in macrophage cultures after infection. Each value represents the mean ± SEM of triplicate cultures from two independent experiments. Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) compared to control group (macrophages that were treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>†</sup> represents significant difference (<sup>†</sup>p<0.05, <sup>†††</sup>p<0.001) compared to IFN-γ control group (macrophages that were activated with IFN-γ, treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>‡</sup> represents significant difference (<sup>‡‡</sup>p<0.01) between the same experimental group compared to different culture conditions.</p>
2013-08-22 02:14:54
immunology
microbiology
cyslt-receptor
antagonists
macrophage
phagocytosis
fungicidal