Effect of LTB<sub>4</sub>- or cysLT-receptor antagonists on macrophage phagocytosis and fungicidal activity against <i>P. brasiliensis</i>. Patrícia Campi Santos Daniel Assis Santos Lucas Secchim Ribeiro Caio Tavares Fagundes Talles Prosperi de Paula Thiago Vinícius Avila Ludmila de Matos Baltazar Mila Moreira Madeira Rosana de Carvalho Cruz Ana Carolina Fialho Dias Fabiana Simão Machado Mauro Martins Teixeira Patrícia Silva Cisalpino Danielle G. Souza 10.1371/journal.pntd.0002390.g006 https://plos.figshare.com/articles/figure/_Effect_of_LTB_4_or_cysLT_receptor_antagonists_on_macrophage_phagocytosis_and_fungicidal_activity_against_P_brasiliensis_/780329 <p>Peritoneal WT macrophages were treated with vehicle, LTB<sub>4</sub> receptor antagonist CP-105,696 (CP) (1 mM) or cysteinyl-LT receptor antagonist montelukast (MK) (10 mM) for 30 min before the addition of <i>P. brasiliensis</i> yeasts. Phagocytic index (A) and intracellular killing (B) were calculated 24 h after incubation. Killing assay was normalized according initial ingestion (PI) and presented as <i>P. brasiliensis</i> survival. The supernatants were harvested and nitrite concentrations (C) were measured. (D) ROS concentrations were determined by mean fluorescence intensity (DHR-123) in macrophage cultures after infection. Each value represents the mean ± SEM of triplicate cultures from two independent experiments. Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) compared to control group (macrophages that were treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>†</sup> represents significant difference (<sup>†</sup>p<0.05, <sup>†††</sup>p<0.001) compared to IFN-γ control group (macrophages that were activated with IFN-γ, treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>‡</sup> represents significant difference (<sup>‡‡</sup>p<0.01) between the same experimental group compared to different culture conditions.</p> 2013-08-22 02:14:54 immunology microbiology cyslt-receptor antagonists macrophage phagocytosis fungicidal