TY - DATA T1 - Effect of LT-synthesis blockade in vivo during infection with P. brasiliensis. PY - 2013/08/22 AU - Patrícia Campi Santos AU - Daniel Assis Santos AU - Lucas Secchim Ribeiro AU - Caio Tavares Fagundes AU - Talles Prosperi de Paula AU - Thiago Vinícius Avila AU - Ludmila de Matos Baltazar AU - Mila Moreira Madeira AU - Rosana de Carvalho Cruz AU - Ana Carolina Fialho Dias AU - Fabiana Simão Machado AU - Mauro Martins Teixeira AU - Patrícia Silva Cisalpino AU - Danielle G. Souza UR - https://plos.figshare.com/articles/figure/_Effect_of_LT_synthesis_blockade_in_vivo_during_infection_with_P_brasiliensis_/780327 DO - 10.1371/journal.pntd.0002390.g004 L4 - https://ndownloader.figshare.com/files/1179702 KW - immunology KW - microbiology KW - lt-synthesis KW - blockade N2 - WT mice were treated with zileuton (30 mg/Kg/day; 0.2 ml by gavage) before infection with P. brasiliensis yeasts. (A) MPO activity in the lungs of mice inoculated with P. brasiliensis yeasts was expressed in relative units. Control (NI) mice were injected with PBS and treated daily with the same volume of vehicle solution by the same route. BAL cells were obtained from mice 24 and 72 h after infection and neutrophils (B) and mononuclear cells (C) were counted and identified after staining. (D) Fungal burden was evaluated by counting the number of viable yeasts presents in BAL (left) and lung homogenates (right). The results were expressed as the mean ± SEM (n = 5). Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) between infected mice compared to respective control group. Symbol † represents significant difference (†p<0.05, ††p<0.01, †††p<0.001) to infected mice counterparts in evaluated periods. Symbol ‡ represents significant difference (‡‡p<0.01, ‡‡‡p<0.001) between WT and 5-LO−/− mice in the respective periods evaluated. ND, not detected. ER -