TY - DATA T1 - Impact on pgRNA encapsidation of selected mutants displaying reduced DNA accumulation. PY - 2013/08/20 AU - Hui Feng AU - Ping Chen AU - Fei Zhao AU - Michael Nassal AU - Kanghong Hu UR - https://plos.figshare.com/articles/figure/_Impact_on_pgRNA_encapsidation_of_selected_mutants_displaying_reduced_DNA_accumulation_/777058 DO - 10.1371/journal.pone.0072798.g005 L4 - https://ndownloader.figshare.com/files/1175805 KW - Biochemistry KW - Nucleic acids KW - rna KW - microbiology KW - Virology KW - Viral replication KW - Viral nucleic acid KW - Viral packaging KW - Viral replication complex KW - Viral enzymes KW - Gastroenterology and hepatology KW - Liver diseases KW - Infectious hepatitis KW - Hepatitis B KW - pgrna KW - encapsidation KW - mutants KW - displaying KW - reduced KW - dna N2 - Total cytoyplasmic RNA and capsid-associated RNA from cells transfected with the indicated constructs were analyzed by RNase protection assays via hybridization to a 314 nucleotide antisense riboprobe (indicated by an asterisk) containing 41 nucleotides of non-HBV sequence; RNase digestion is expected to yield a protected fragment of about 270 nt (indicated by the arrow). Numbers below each lane show the encapsidation efficiency, measured as the ratio of encapsidated versus total pgRNA, relative to that produced by the wild-type HBV construct which was set to 100. Mean values ± standard deviation are from three independent experiments. ER -