Ad-IL-17AR:Fc administration reduces IL-17A production in the blood and the heart. Yuquan Xie Minghui Li Xinggang Wang Xian Zhang Tianqing Peng Yingzhen Yang Yunzeng Zou Junbo Ge Haozhu Chen Ruizhen Chen 10.1371/journal.pone.0072158.g001 https://plos.figshare.com/articles/figure/_Ad_IL_17AR_Fc_administration_reduces_IL_17A_production_in_the_blood_and_the_heart_/777052 <p>(<i>A</i>) Serum IL-6 concentrations at post-infection 7 days, 2mons and 3mons in PBS-, Ad-IL-17AR:Fc- and Ad:null-treated mice (n = 5) were measured using ELISA, and the data were analyzed by a 2-factor ANOVA. <sup>##</sup><i>p</i><0.01, <sup>#</sup><i>p</i><0.05 vs 7 Days group, **<i>p</i><0.01, *<i>p</i><0.05 vs control group, (<i>B</i>) Serum TNF-α concentrations at post-infection 7 days, 2mons and 3mons in PBS-, AdIL-17R:Fc- and Ad:null-treated mice (n = 5) were measured using ELISA, and the data were analyzed by a 2-factor ANOVA. <sup>#</sup><i>p</i><0.05 vs 7 Days group, *<i>p</i><0.05 vs control group. The data represent the mean ± SEM for 6 mice per group. (<i>C</i>)The IL-17A protein extracted from the left ventricle of the mice in the three groups at post-infection day 90, was subjected to western blotting and was probed with the indicated antibodies (n = 3). (D)The IL-17A protein level is expressed as a ratio of the GAPDH level in the same sample; the data are expressed as the mean± SEM. *<i>P</i><0.05 vs the control group. (<i>E</i>) The IL-17RA protein extracted from the left ventricle of the mice in the three groups at post-infection day 90, was subjected to western blotting and was probed with the indicated antibodies (n = 3). (<i>F</i>) The IL-17RA protein level is expressed as a ratio of the GAPDH level in the same sample; the data are expressed as the mean± SEM.</p> 2013-08-20 01:41:39 immunology Immune cells t cells Immune system cytokines microbiology Vector biology Viral vectors Model organisms Animal models mouse Molecular cell biology Extracellular matrix cardiovascular cardiomyopathies Interventional cardiology Clinical immunology Infectious diseases Viral diseases toxicology Immunotoxicology il-17a