Ad-IL-17AR:Fc administration reduces IL-17A production in the blood and the heart.
Yuquan Xie
Minghui Li
Xinggang Wang
Xian Zhang
Tianqing Peng
Yingzhen Yang
Yunzeng Zou
Junbo Ge
Haozhu Chen
Ruizhen Chen
10.1371/journal.pone.0072158.g001
https://plos.figshare.com/articles/figure/_Ad_IL_17AR_Fc_administration_reduces_IL_17A_production_in_the_blood_and_the_heart_/777052
<p>(<i>A</i>) Serum IL-6 concentrations at post-infection 7 days, 2mons and 3mons in PBS-, Ad-IL-17AR:Fc- and Ad:null-treated mice (n = 5) were measured using ELISA, and the data were analyzed by a 2-factor ANOVA. <sup>##</sup><i>p</i><0.01, <sup>#</sup><i>p</i><0.05 vs 7 Days group, **<i>p</i><0.01, *<i>p</i><0.05 vs control group, (<i>B</i>) Serum TNF-α concentrations at post-infection 7 days, 2mons and 3mons in PBS-, AdIL-17R:Fc- and Ad:null-treated mice (n = 5) were measured using ELISA, and the data were analyzed by a 2-factor ANOVA. <sup>#</sup><i>p</i><0.05 vs 7 Days group, *<i>p</i><0.05 vs control group. The data represent the mean ± SEM for 6 mice per group. (<i>C</i>)The IL-17A protein extracted from the left ventricle of the mice in the three groups at post-infection day 90, was subjected to western blotting and was probed with the indicated antibodies (n = 3). (D)The IL-17A protein level is expressed as a ratio of the GAPDH level in the same sample; the data are expressed as the mean± SEM. *<i>P</i><0.05 vs the control group. (<i>E</i>) The IL-17RA protein extracted from the left ventricle of the mice in the three groups at post-infection day 90, was subjected to western blotting and was probed with the indicated antibodies (n = 3). (<i>F</i>) The IL-17RA protein level is expressed as a ratio of the GAPDH level in the same sample; the data are expressed as the mean± SEM.</p>
2013-08-20 01:41:39
immunology
Immune cells
t cells
Immune system
cytokines
microbiology
Vector biology
Viral vectors
Model organisms
Animal models
mouse
Molecular cell biology
Extracellular matrix
cardiovascular
cardiomyopathies
Interventional cardiology
Clinical immunology
Infectious diseases
Viral diseases
toxicology
Immunotoxicology
il-17a