10.1371/journal.pone.0072281.g001 Barbara Costa Barbara Costa Sara Bendinelli Sara Bendinelli Pamela Gabelloni Pamela Gabelloni Eleonora Da Pozzo Eleonora Da Pozzo Simona Daniele Simona Daniele Fabrizio Scatena Fabrizio Scatena Renato Vanacore Renato Vanacore Pietro Campiglia Pietro Campiglia Alessia Bertamino Alessia Bertamino Isabel Gomez-Monterrey Isabel Gomez-Monterrey Daniela Sorriento Daniela Sorriento Carmine Del Giudice Carmine Del Giudice Guido Iaccarino Guido Iaccarino Ettore Novellino Ettore Novellino Claudia Martini Claudia Martini ISA27 inhibits the growth/survival of GBM cell lines expressing wild-type p53. Public Library of Science 2013 Biochemistry drug discovery Small molecules Molecular cell biology Cell death Drugs and devices Drug research and development oncology Cancers and neoplasms Neurological tumors Glioblastoma multiforme Basic cancer research inhibits gbm lines expressing wild-type 2013-08-19 03:55:18 Figure https://plos.figshare.com/articles/figure/_ISA27_inhibits_the_growth_survival_of_GBM_cell_lines_expressing_wild_type_p53_/776367 <p>GBM cells with wild-type p53 (U87MG, U343MG) were exposed to a range of ISA27 or Nutlin-3 concentrations for 24 h. After incubation, GBM cell viability was determined by MTS assay. <i>A and B) Effect of ISA27 on the growth/survival of GBM cell lines expressing wild-type p53:</i> curves for U87MG and U343MG cell samples were generated using a sigmoidal dose-response curve model (GraphPad Prism 4 software) from which the IC<sub>50</sub> values were derived. ISA27-treated cells (▾), Nutlin-3-treated cells (▪); <i>C) Effect of ISA27 on the growth/survival of the GBM T98G cell line expressing mutant p53:</i> T98G cells were incubated with increasing concentrations of ISA27 or Nutlin-3 (ranging from 1 µM to 25 µM), and cell viability was measured after 24 h by MTS assay. ISA27-treated cells (<b>▾</b>), Nutlin-3-treated cells (<b>▪</b>). The results are expressed as the percentages of cell viability with respect to the vehicle-treated sample for each cell line; the viability of the vehicle-treated sample was arbitrarily set at 100%. Points, means of three independent experiments performed in duplicate; bars, SEM.</p>