10.1371/journal.pone.0072281.g001
Barbara Costa
Barbara
Costa
Sara Bendinelli
Sara
Bendinelli
Pamela Gabelloni
Pamela
Gabelloni
Eleonora Da Pozzo
Eleonora Da
Pozzo
Simona Daniele
Simona
Daniele
Fabrizio Scatena
Fabrizio
Scatena
Renato Vanacore
Renato
Vanacore
Pietro Campiglia
Pietro
Campiglia
Alessia Bertamino
Alessia
Bertamino
Isabel Gomez-Monterrey
Isabel
Gomez-Monterrey
Daniela Sorriento
Daniela
Sorriento
Carmine Del Giudice
Carmine
Del Giudice
Guido Iaccarino
Guido
Iaccarino
Ettore Novellino
Ettore
Novellino
Claudia Martini
Claudia
Martini
ISA27 inhibits the growth/survival of GBM cell lines expressing wild-type p53.
Public Library of Science
2013
Biochemistry
drug discovery
Small molecules
Molecular cell biology
Cell death
Drugs and devices
Drug research and development
oncology
Cancers and neoplasms
Neurological tumors
Glioblastoma multiforme
Basic cancer research
inhibits
gbm
lines
expressing
wild-type
2013-08-19 03:55:18
Figure
https://plos.figshare.com/articles/figure/_ISA27_inhibits_the_growth_survival_of_GBM_cell_lines_expressing_wild_type_p53_/776367
<p>GBM cells with wild-type p53 (U87MG, U343MG) were exposed to a range of ISA27 or Nutlin-3 concentrations for 24 h. After incubation, GBM cell viability was determined by MTS assay. <i>A and B) Effect of ISA27 on the growth/survival of GBM cell lines expressing wild-type p53:</i> curves for U87MG and U343MG cell samples were generated using a sigmoidal dose-response curve model (GraphPad Prism 4 software) from which the IC<sub>50</sub> values were derived. ISA27-treated cells (▾), Nutlin-3-treated cells (▪); <i>C) Effect of ISA27 on the growth/survival of the GBM T98G cell line expressing mutant p53:</i> T98G cells were incubated with increasing concentrations of ISA27 or Nutlin-3 (ranging from 1 µM to 25 µM), and cell viability was measured after 24 h by MTS assay. ISA27-treated cells (<b>▾</b>), Nutlin-3-treated cells (<b>▪</b>). The results are expressed as the percentages of cell viability with respect to the vehicle-treated sample for each cell line; the viability of the vehicle-treated sample was arbitrarily set at 100%. Points, means of three independent experiments performed in duplicate; bars, SEM.</p>