Immunohistochemical detection of FGFR-2-IIIb in thyroid tissue. Adriano Redler Giorgio Di Rocco Domenico Giannotti Francesca Frezzotti Maria Giulia Bernieri Simona Ceccarelli Sirio D’Amici Enrica Vescarelli Anna Paola Mitterhofer Antonio Angeloni Cinzia Marchese 10.1371/journal.pone.0072224.g002 https://plos.figshare.com/articles/figure/_Immunohistochemical_detection_of_FGFR_2_IIIb_in_thyroid_tissue_/775842 <p>Dewaxed sections of normal thyroid tissue (A, A’), hyperplastic thyroid tissue (B, B’), follicular adenoma (C, C’) and papillary carcinoma (D, D’) were subjected to immunohistochemistry using a home-made antibody directed against FGFR-2-IIIb. (<b>A–D</b>), original magnification 10×, scale bar 20 µm. A significant area in each panel is indicated by a square and an enlargement of this area is shown in the respective (<b>A’–D’</b>) panels (original magnification 20×, scale bar 20 µm). (<b>A–A’</b>) Normal thyroid sections showing positive staining pattern for FGFR-2-IIIb in both cytoplasm (arrow) and cell membrane compartment (arrowhead) of follicular cells. (<b>B–B’</b>) Hyperplastic thyroid sections showing positive staining pattern for FGFR-2-IIIb in follicular cells cytoplasm (arrows). (<b>C–C’</b>)<b>,</b> (<b>D–D’</b>) Follicular adenoma and papillary carcinoma samples showing no FGFR-2-IIIb signal in follicular cells (arrows).</p> 2013-08-19 02:36:40 Anatomy and physiology Endocrine system Endocrine physiology Growth factors Biochemistry proteins Diagnostic medicine Endocrinology oncology Cancers and neoplasms Endocrine tumors detection fgfr-2-iiib thyroid