10.1371/journal.pone.0071448.g002
Mikhail V. Matz
Mikhail
V. Matz
Rachel M. Wright
Rachel
M. Wright
James G. Scott
James
G. Scott
Effect of <i>Cq</i>1 setting on the point estimates and credible intervals of the fixed effects of stress in the coral dataset, according to the naive model.
Public Library of Science
2013
Biochemistry
Nucleic acids
dna
DNA amplification
Computational biology
Molecular genetics
gene expression
genetics
genomics
Functional genomics
Marine biology
corals
Molecular cell biology
estimates
credible
intervals
coral
naive
2013-08-19 02:36:51
Figure
https://plos.figshare.com/articles/figure/_Effect_of_Cq_1_setting_on_the_point_estimates_and_credible_intervals_of_the_fixed_effects_of_stress_in_the_coral_dataset_according_to_the_naive_model_/775814
<p>The points are posterior means, the 95% credible intervals are denoted as dashed lines connecting upper and lower interval limits across genes, to better visualize changes in their width. (a) Comparison of the results based on formula-approximated <i>Cq</i>1 (“est.”) with analyses assuming the same inflated (40) or diminished (35) <i>Cq</i>1 for all genes. The three most affected genes are <i>clect</i>, <i>chrom</i>, <i>g3pdh</i>, and <i>hsp16</i>, which were so low-abundant in at least one of the experimental conditions such that many of their qPCR trials were empty. (b) Comparison between analyses with formula-based <i>Cq</i>1 (“est.”) and uniform <i>Cq</i>1 = 37 for all genes.</p>