Sayed, Mahmoud Pyo, Sang-Hyun Rehnberg, Nicola Hatti-Kaul, Rajni Selective Oxidation of 5‑Hydroxymethylfurfural to 5‑Hydroxymethyl-2-furancarboxylic Acid Using <i>Gluconobacter oxydans</i> 5-Hydroxymethylfurfural (HMF), an important biobased platform chemical, can be potentially oxidized to several products that can serve as versatile building blocks for polymers. 5-Hydroxymethyl-2-furan carboxylic acid (HMFCA) is formed by incomplete oxidation of HMF but the reaction often suffers from substrate inhibition and overoxidation to other products. In this study, resting cells of <i>Gluconobacteroxydans</i> DSM 50049 were shown to oxidize HMF quantitatively to HMFCA with exquisite selectivity. Complete conversion of 31.5 g L<sup>–1</sup> crude HMF to HMFCA was achieved within 6 h under pH-controlled conditions. Initial productivity of 10 g L<sup>–1</sup> h<sup>–1</sup> was reduced to 2 g L<sup>–1</sup> h<sup>–1</sup> toward the end of the reaction. Thereafter, additional HMF added to the reaction mixture (12 g L<sup>–1</sup>) was converted up to 94% within 17 h with 100% selectivity resulting in final HMFCA concentration of 44.6 g L<sup>–1</sup> and yield of 6.2 g g<sup>–1</sup> cell dry weight. Recovery of HMFCA from the reaction could be achieved by adsorption to anion exchange resins Amberlite IRA-400 (Cl<sup>–</sup> form) and Ambersep 900 (OH<sup>–</sup> form), the former showing higher binding (169 mg/g resin) and product recovery. Alternatively, liquid–liquid extraction with ethyl acetate provided a facile separation technique for the recovery of pure HMFCA. separation technique;Gluconobacter oxydans 5- Hydroxymethylfurfural;17 h;OH;Complete conversion;Ambersep 900;Gluconobacteroxydans DSM 50049;pH-controlled conditions;ethyl acetate;5- Hydroxymethyl -2-furan carboxylic acid;substrate inhibition;HMFCA concentration;building blocks;oxidize HMF;biobased platform chemical;product recovery;Initial productivity;anion exchange resins Amberlite IRA -400;Selective Oxidation;6 h 2019-01-25
    https://acs.figshare.com/articles/journal_contribution/Selective_Oxidation_of_5_Hydroxymethylfurfural_to_5_Hydroxymethyl-2-furancarboxylic_Acid_Using_i_Gluconobacter_oxydans_i_/7692194
10.1021/acssuschemeng.8b06327.s001