DSB-2 and SUN-1 S8P persist when DSB formation is defective. Simona Rosu Karl A. Zawadzki Ericca L. Stamper Diana E. Libuda Angela L. Reese Abby F. Dernburg Anne M. Villeneuve 10.1371/journal.pgen.1003674.g005 https://plos.figshare.com/articles/figure/_DSB_2_and_SUN_1_S8P_persist_when_DSB_formation_is_defective_/767726 <p>(A) and (B) Immunofluorescence images of gonads from the distal pre-meiotic region to end of pachytene, stained with DAPI and antibodies that recognize DSB-2 and SUN-1 S8P. The zone of DSB-2 and SUN-1 S8P-positive nuclei is extended in both <i>spo-11</i> (A) and <i>him-17</i> (B) mutants, which are defective in DSB formation. (C) Close-up images of fields of nuclei in early pachytene, as outlined in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003674#pgen-1003674-g003" target="_blank">Figure 3A</a> and (A), (B) above. WT as well as <i>spo-11</i> nuclei show bright patches of DSB-2 staining, whereas <i>him-17</i> nuclei do not. Scale bar, 15 µm.</p> 2013-08-08 02:43:31 sun-1 s8p dsb