TY - DATA T1 - Fsc1 localizes to the vacuole membrane and forms starvation-induced puncta. PY - 2013/08/08 AU - Ling-Ling Sun AU - Ming Li AU - Fang Suo AU - Xiao-Man Liu AU - En-Zhi Shen AU - Bing Yang AU - Meng-Qiu Dong AU - Wan-Zhong He AU - Li-Lin Du UR - https://plos.figshare.com/articles/figure/_Fsc1_localizes_to_the_vacuole_membrane_and_forms_starvation_induced_puncta_/767716 DO - 10.1371/journal.pgen.1003715.g007 L4 - https://ndownloader.figshare.com/files/1147259 KW - genetics KW - Genetic screens KW - genomics KW - Functional genomics KW - Model organisms KW - Yeast and fungal models KW - Molecular cell biology KW - Membranes and sorting KW - localizes KW - vacuole KW - membrane KW - forms KW - starvation-induced N2 - (A) The predicted membrane topology and domain organization of Fsc1. (B) Localization of Fsc1 in non-starved cells. Cpy1 and Zhf1 are vacuole lumen and vacuole membrane markers, respectively. Bar, 3 µm. (C) Localization of Fsc1 in starved cells. Bar, 6 µm. (D) Time-lapse images of Fsc1 puncta induced by starvation. Bar, 3 µm. (E) Fsc1 puncta induced by starvation are dependent on Atg1, Atg11, and partially dependent on Atg13. Two hundred cells were examined for each data point. ER -