Time-course analysis of L1 and L5 internalization by human aortic endothelial cells (HAECs) and L5-induced C-reactive protein (CRP) expression. Chih-Sheng Chu Yu-Chen Wang Long-Sheng Lu Brian Walton H. Ramazan Yilmaz Roger Y. Huang Tatsuya Sawamura Richard A. F. Dixon Wen-Ter Lai Chu-Huang Chen Jonathan Lu 10.1371/journal.pone.0070533.g005 https://plos.figshare.com/articles/figure/_Time_course_analysis_of_L1_and_L5_internalization_by_human_aortic_endothelial_cells_HAECs_and_L5_induced_C_reactive_protein_CRP_expression_/767715 <p>(A) Results of fluorescence microscopy showing that DiI-L1 and DiI-L5 (each red) were internalized by HAECs at different time points. The nuclei of HAECs were co-stained with 4ā€²,6-diamidino-2-phenylindole (DAPI) (blue). (B) Western blot showing that L5 augmented endothelial CRP expression in a time-dependent manner. Time point 0 represents the phosphate-buffered saline (PBS) control. *<i>P</i><0.05 vs. PBS-treated control. (C) L5 deprivation study showed that internalized L5 continued to induce CRP and lectin-like oxidized receptor-1 (LOX-1) expression 24 hours after replacing L5 conditioned culture media with fresh EGM2 media. L5 exposure times are shown. HAECs were incubated with recombinant CRP for 2 hours as a positive control. Time point 0 represents the PBS control. For all experiments, nā€Š=ā€Š3, and bars in graphs represent standard deviation. ā€ <i>P</i><0.05 vs. PBS-treated CRP control, *<i>P</i><0.05 vs. PBS-treated LOX-1 control.</p> 2013-08-08 02:41:45 Biochemistry proteins lipoproteins Lipoprotein receptors Molecular cell biology chromatography cardiovascular atherosclerosis Diagnostic medicine l1 l5 internalization aortic endothelial cells l5-induced c-reactive