Detection of total reactive oxygen species (ROS) in human aortic endothelial cells (HAECs). Chih-Sheng Chu Yu-Chen Wang Long-Sheng Lu Brian Walton H. Ramazan Yilmaz Roger Y. Huang Tatsuya Sawamura Richard A. F. Dixon Wen-Ter Lai Chu-Huang Chen Jonathan Lu 10.1371/journal.pone.0070533.g003 https://plos.figshare.com/articles/figure/_Detection_of_total_reactive_oxygen_species_ROS_in_human_aortic_endothelial_cells_HAECs_/767712 <p>(A) L5 (50 µg/mL) from patients increased ROS production (green) in HAECs after 20 minutes (top and middle row) and after 24 hours (bottom row). Compared to the phosphate-buffered saline (PBS) control, L1 treatment had no effect. Staining with 4′,6-diamidino-2-phenylindole (DAPI) (blue) indicated HAEC apoptosis 24 hours after L5 (50 µg/mL) exposure. The pretreatment of cells with TS92 attenuated the L5-induced ROS increase after 20 minutes (middle row). (B) Blocking the production of ROS by adding ROS inhibitor N-acetyl cysteine (NAC) attenuated the L5-induced increase in CRP levels. For all experiments, n = 3, and bars shown in graphs represent standard deviation. *<i>P</i><0.05, **<i>P</i><0.01 vs. untreated control.</p> 2013-08-08 02:41:45 Biochemistry proteins lipoproteins Lipoprotein receptors Molecular cell biology chromatography cardiovascular atherosclerosis Diagnostic medicine reactive aortic endothelial cells