Analysis of C-reactive protein (CRP) expression by using Western blot and enzyme-linked immunosorbent assay (ELISA).
Chih-Sheng Chu
Yu-Chen Wang
Long-Sheng Lu
Brian Walton
H. Ramazan Yilmaz
Roger Y. Huang
Tatsuya Sawamura
Richard A. F. Dixon
Wen-Ter Lai
Chu-Huang Chen
Jonathan Lu
10.1371/journal.pone.0070533.g002
https://plos.figshare.com/articles/figure/_Analysis_of_C_reactive_protein_CRP_expression_by_using_Western_blot_and_enzyme_linked_immunosorbent_assay_ELISA_/767710
<p>(A, B) C-reactive protein expression in human aortic endothelial cells (HAECs) treated with L5 from 5 hypercholesterolemic patients (patients labeled as A–E). L5 increased endothelial CRP expression by up to 2.5-fold more than did L1. (C) L5 treatment (10–100 µg/mL) increased endothelial CRP expression in a dose-dependent manner. (D) ELISA analysis of CRP in the conditioned culture medium (CCM) of HAECs treated with phosphate-buffered saline (PBS), L1, or L5. For all experiments, n = 3, and bars shown in graphs represent standard deviation. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 vs. PBS or untreated control.</p>
2013-08-08 02:41:45
Biochemistry
proteins
lipoproteins
Lipoprotein receptors
Molecular cell biology
chromatography
cardiovascular
atherosclerosis
Diagnostic medicine
c-reactive
blot
enzyme-linked
immunosorbent
assay