Forced expression of A1 restores the survival of OX40 KO CD8 T cells in secondary responses <i>in vitro</i>. Fengyang Lei Jianyong Song Rizwanul Haque Mohammad Haque Xiaofang Xiong Deyu Fang Michael Croft Jianxun Song 10.1371/journal.pone.0070635.g004 https://plos.figshare.com/articles/figure/_Forced_expression_of_A1_restores_the_survival_of_OX40_KO_CD8_T_cells_in_secondary_responses_in_vitro_/761926 <p>Naïve CD8 cells from WT or OX40 KO OT-I TCR transgenic mice were stimulated with peptide and APCs. On day 2/3, T cells were transduced with retroviral vectors expressing GFP, or GFP with A1. On day 5 of primary culture, GFP<sup>+</sup> CD8 T cells were sorted, and restimulated with APCs and peptide. (a) Recall survival, based on recovery of GFP<sup>+</sup>Vβ5<sup>+</sup> T cells over time. Cell numbers present on day 0 were assigned a value of 100%, and cell numbers surviving on day 2, day 4 and day 6 were used to calculate the percentage recovery. Data represent the mean ± s.d. percentage changes from three separate experiments (<i>p</i>>0.05). (b) Recall IL-2 and IFN-γ production by ELISA at 40 h. Data are means ± s.d. from three experiments (<i>p</i>>0.05).</p> 2013-08-01 03:02:18 immunology Immune cells t cells Immune response Molecular cell biology Cell death Clinical immunology oncology Cancer treatment immunotherapy a1 restores ox40 ko cd8 cells responses