%0 Figure %A Bandyopadhyay, Sanghamitra %A Cahill, Catherine %A Balleidier, Amelie %A Huang, Conan %A K. Lahiri, Debomoy %A Huang, Xudong %A T. Rogers, Jack %D 2013 %T Alignment of human and mouse APP 5′UTRs with human PrP 5′UTR sequences relative to the L- and H-ferritin Iron-responsive elements (IREs). %U https://plos.figshare.com/articles/figure/_Alignment_of_human_and_mouse_APP_5_8242_UTRs_with_human_PrP_5_8242_UTR_sequences_relative_to_the_L_and_H_ferritin_Iron_responsive_elements_IREs_/760759 %R 10.1371/journal.pone.0065978.g001 %2 https://ndownloader.figshare.com/files/1138352 %K Biochemistry %K Nucleic acids %K rna %K proteins %K genetics %K Molecular cell biology %K Cellular types %K neurons %K gene expression %K Protein translation %K nanotechnology %K ferritin %K Clinical genetics %K Chromosomal disorders %K Down syndrome %K neurology %K dementia %K Alzheimer disease %K app %K prp %K sequences %K l- %K h-ferritin %K iron-responsive %K elements %X

Panel A: The human and mouse APP 5′UTR specific IRE-like RNA stem loops, the human PrP 5′UTR, and the human and mouse SNCA specific IRE –like stem loops each aligned adjacent to the ferritin L- and H IRE RNA stem loops. Shown, the L- and H-mRNAs encode canonical IRE RNA stem loops whereas the APP IRE in non canonical although fully iron responsive [6]. The α-synuclein IRE (SNCA IRE) represents a non canonical IRE traversing the central splice junction of exon-1 and exon-2 (the CAGUGN loop/splice site sequences) of SNCA mRNA [49]. Typical IRE stem loops fold to exhibit an apical AGU pseudotriloop which is depicted in red lettering at the apex of the H-ferritin and SNCA IREs [28] relative to an analogous AGA from the IRE–like stem loop encoded by APP mRNA [6]. Panel B: Maps of the 5′UTRs encoding by the human and mouse APP mRNAs, PrP mRNA, SNCA mRNA, and the mRNAs for L- and H-ferritin (IRE stem loops are displayed as lollipops). Panel C: Relative alignment of the sequences that encode the 5′UTR specific IRE-like stem loops in human APP mRNA, PrP mRNA, SNCA mRNA, and the L- and H-ferritin mRNAs. Panel D: Screen and counter-screening Constructs [21]: The human APP 5′UTR cassette was subcloned in front of the luciferase reporter gene in the dicistronic pCD(APP) reporter construct. The same-sized and related PrP 5′UTR was subcloned in an identical format into the pCD(PrP) reporter construct for the purpose of counter-screening, as described in the materials and methods section.

%I PLOS ONE