Alignment of human and mouse APP 5′UTRs with human PrP 5′UTR sequences relative to the L- and H-ferritin Iron-responsive elements (IREs). Sanghamitra Bandyopadhyay Catherine Cahill Amelie Balleidier Conan Huang Debomoy K. Lahiri Xudong Huang Jack T. Rogers 10.1371/journal.pone.0065978.g001 https://plos.figshare.com/articles/figure/_Alignment_of_human_and_mouse_APP_5_8242_UTRs_with_human_PrP_5_8242_UTR_sequences_relative_to_the_L_and_H_ferritin_Iron_responsive_elements_IREs_/760759 <p><b>Panel A</b>: The human and mouse APP 5′UTR specific IRE-like RNA stem loops, the human PrP 5′UTR, and the human and mouse <i>SNCA</i> specific IRE –like stem loops each aligned adjacent to the ferritin L- and H IRE RNA stem loops. Shown, the L- and H-mRNAs encode canonical IRE RNA stem loops whereas the APP IRE in non canonical although fully iron responsive <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#pone.0065978-Cho1" target="_blank">[6]</a>. The α-synuclein IRE (<i>SNCA</i> IRE) represents a non canonical IRE traversing the central splice junction of exon-1 and exon-2 (the CAGUGN loop/splice site sequences) of <i>SNCA</i> mRNA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#pone.0065978-Friedlich1" target="_blank">[49]</a>. Typical IRE stem loops fold to exhibit an apical AGU pseudotriloop which is depicted in red lettering at the apex of the H-ferritin and <i>SNCA</i> IREs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#pone.0065978-Goforth1" target="_blank">[28]</a> relative to an analogous AGA from the IRE–like stem loop encoded by APP mRNA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#pone.0065978-Cho1" target="_blank">[6]</a>. <b>Panel B</b>: Maps of the 5′UTRs encoding by the human and mouse APP mRNAs, PrP mRNA, <i>SNCA</i> mRNA, and the mRNAs for L- and H-ferritin (IRE stem loops are displayed as lollipops). <b>Panel C</b>: Relative alignment of the sequences that encode the 5′UTR specific IRE-like stem loops in human APP mRNA, PrP mRNA, <i>SNCA</i> mRNA, and the L- and H-ferritin mRNAs. <b>Panel D</b>: <u>Screen and counter-screening Constructs </u><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#pone.0065978-Bandyopadhyay2" target="_blank">[<b>21</b>]</a><b>:</b> The human APP 5′UTR cassette was subcloned in front of the luciferase reporter gene in the dicistronic pCD(APP) reporter construct. The same-sized and related PrP 5′UTR was subcloned in an identical format into the pCD(PrP) reporter construct for the purpose of counter-screening, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065978#s2" target="_blank">materials and methods</a> section.</p> 2013-07-31 01:51:32 Biochemistry Nucleic acids rna proteins genetics Molecular cell biology Cellular types neurons gene expression Protein translation nanotechnology ferritin Clinical genetics Chromosomal disorders Down syndrome neurology dementia Alzheimer disease app prp sequences l- h-ferritin iron-responsive elements