TY - DATA T1 - Phosphatase activities of TI loop mutants. PY - 2013/07/29 AU - Kirstin Hobiger AU - Tillmann Utesch AU - Maria Andrea Mroginski AU - Guiscard Seebohm AU - Thomas Friedrich UR - https://plos.figshare.com/articles/figure/_Phosphatase_activities_of_TI_loop_mutants_/759325 DO - 10.1371/journal.pone.0070272.g004 L4 - https://ndownloader.figshare.com/files/1136412 KW - Anatomy and physiology KW - electrophysiology KW - biophysics KW - Biophysics simulations KW - Computational biology KW - Macromolecular structure analysis KW - protein structure KW - Molecular cell biology KW - Signal transduction KW - Signaling cascades KW - Phospholipid signaling cascade KW - Polyphosphoinositide signaling cascade KW - Signaling in cellular processes KW - Lipid signaling KW - Transmembrane signaling KW - Signaling in selected disciplines KW - Neurological signaling KW - Oncogenic signaling KW - Signaling pathways KW - Phosphoinositide signal transduction KW - Mechanisms of signal transduction KW - neuroscience KW - Cellular neuroscience KW - ion channels KW - activities KW - ti N2 - (A) Representative currents traces of Ci-VSP WT and denoted mutants that were co-expressed with KCNQ2/KCNQ3 potassium channels in Xenopus oocytes. Channel currents were recorded in response to a depolarization pulse from a holding potential of −80 to+80 mV for the indicated time interval. (B–C) From the resulting current traces, maximal and minimal current amplitudes (Imax and Imin) were determined during the depolarization phase at+80 mV. Inhibition ratios calculated with the values of Imin and Imax are plotted with the corresponding time durations required for each mutant to inhibit the channel currents to 50% (τ50%). Inhibition ratio of 0.5 (corresponding to 50%) is highlighted as dashed line. ER -