10.1371/journal.pone.0070272.g004
Kirstin Hobiger
Kirstin
Hobiger
Tillmann Utesch
Tillmann
Utesch
Maria Andrea Mroginski
Maria Andrea
Mroginski
Guiscard Seebohm
Guiscard
Seebohm
Thomas Friedrich
Thomas
Friedrich
Phosphatase activities of TI loop mutants.
Public Library of Science
2013
Anatomy and physiology
electrophysiology
biophysics
Biophysics simulations
Computational biology
Macromolecular structure analysis
protein structure
Molecular cell biology
Signal transduction
Signaling cascades
Phospholipid signaling cascade
Polyphosphoinositide signaling cascade
Signaling in cellular processes
Lipid signaling
Transmembrane signaling
Signaling in selected disciplines
Neurological signaling
Oncogenic signaling
Signaling pathways
Phosphoinositide signal transduction
Mechanisms of signal transduction
neuroscience
Cellular neuroscience
ion channels
activities
ti
2013-07-29 07:32:35
Figure
https://plos.figshare.com/articles/figure/_Phosphatase_activities_of_TI_loop_mutants_/759325
<p><i>(</i><b><i>A</i></b><i>)</i> Representative currents traces of Ci-VSP WT and denoted mutants that were co-expressed with KCNQ2/KCNQ3 potassium channels in <i>Xenopus</i> oocytes. Channel currents were recorded in response to a depolarization pulse from a holding potential of −80 to+80 mV for the indicated time interval. <i>(</i><b><i>B</i></b><b>–</b><b><i>C</i></b><i>)</i> From the resulting current traces, maximal and minimal current amplitudes (I<sub>max</sub> and I<sub>min</sub>) were determined during the depolarization phase at+80 mV. Inhibition ratios calculated with the values of I<sub>min</sub> and I<sub>max</sub> are plotted with the corresponding time durations required for each mutant to inhibit the channel currents to 50% (τ<sub>50%</sub>). Inhibition ratio of 0.5 (corresponding to 50%) is highlighted as dashed line.</p>