10.1371/journal.pone.0070272.g004 Kirstin Hobiger Kirstin Hobiger Tillmann Utesch Tillmann Utesch Maria Andrea Mroginski Maria Andrea Mroginski Guiscard Seebohm Guiscard Seebohm Thomas Friedrich Thomas Friedrich Phosphatase activities of TI loop mutants. Public Library of Science 2013 Anatomy and physiology electrophysiology biophysics Biophysics simulations Computational biology Macromolecular structure analysis protein structure Molecular cell biology Signal transduction Signaling cascades Phospholipid signaling cascade Polyphosphoinositide signaling cascade Signaling in cellular processes Lipid signaling Transmembrane signaling Signaling in selected disciplines Neurological signaling Oncogenic signaling Signaling pathways Phosphoinositide signal transduction Mechanisms of signal transduction neuroscience Cellular neuroscience ion channels activities ti 2013-07-29 07:32:35 Figure https://plos.figshare.com/articles/figure/_Phosphatase_activities_of_TI_loop_mutants_/759325 <p><i>(</i><b><i>A</i></b><i>)</i> Representative currents traces of Ci-VSP WT and denoted mutants that were co-expressed with KCNQ2/KCNQ3 potassium channels in <i>Xenopus</i> oocytes. Channel currents were recorded in response to a depolarization pulse from a holding potential of −80 to+80 mV for the indicated time interval. <i>(</i><b><i>B</i></b><b>–</b><b><i>C</i></b><i>)</i> From the resulting current traces, maximal and minimal current amplitudes (I<sub>max</sub> and I<sub>min</sub>) were determined during the depolarization phase at+80 mV. Inhibition ratios calculated with the values of I<sub>min</sub> and I<sub>max</sub> are plotted with the corresponding time durations required for each mutant to inhibit the channel currents to 50% (τ<sub>50%</sub>). Inhibition ratio of 0.5 (corresponding to 50%) is highlighted as dashed line.</p>